NsiI from New England Biolabs

Product Specs
ItemNsiI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0127L
Quantity5000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

ATGCA/T

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Engineering heterologous enzyme secretion in Yarrowia lipolyticaMicrobial Cell FactoriesDecember 1, 2022Weigao Wang, Mark A. Blennerverification of PAH1 knockout strain To construct the CRISPR knockout vectors, NsiI (Catalog number: R0127S, NEB) was used to digest parent pCas9-Leu or pCas9-URA plasmids [29]. Corresponding primers were used

(2) STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cellsCell Rep MethodsOctober 24, 2022Blanch-Asensio A, Grandela C, Brandão KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, et al.,New England Biolabs,Cat#R0104S EcoRI,New England Biolabs,Cat#R0101S PvuI,New England Biolabs,Cat#R0150S NsiI,New England Biolabs,Cat#R0127S Esp3I,New England Biolabs,Cat#R0734S HaeIII,New England Biolabs,Cat#R0108S MseI,New England Biolabs,Cat#R0525S BpiI,ThermoFisher,Cat#ER1011 FastDigest Eco31I

(3) Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattleBMC BiotechnologyDecember 1, 2021R. R. Alyethodi, U. Singh, S. Kumar, R. Alex, G. S. Sengar, T. V. Raja, R. Deb, B. PrakashPCR was validated by our earlier published method of PCR-PIRA [4] using the NsiI enzyme (NEB India, R0127S). For further comparison, 100 samples which were earlier tested for CVM by PCRPIRA [22] were added and

(4) Impact of C‐terminal amino acid composition on protein expression in bacteriaMolecular Systems BiologyMay 25, 2020Marc Weber, Raul Burgos, Eva Yus, Jae‐Seong Yang, Maria Lluch‐Senar, Luis SerranoONE‐Glo Luciferase Assay System,Promega,E6110 EcoRV,NEB,R0195S XhoI,NEB,R0146S BamHI,NEB,R0136S EcoNI,NEB,R0521S NotI,NEB,R0189S Eco147I,ThermoScientific,FD0424 NsiI,NEB,R0127S T4 ligase,NEB,M0202S Phusion High‐Fidelity DNA Polymerase,ThermoScientific,F530S Chlorampehnicol,Sigma,C0378 Tetracycline,Sigma,T7660 Gentamycin,Sigma,G1397 HEPES,Sigma,H4034 Sucrose,Sigma,84097 Software,Software,Software Python

(5) Population epigenetic divergence exceeds genetic divergence in the Eastern oyster Crassostrea virginica in the Northern Gulf of MexicoEvolutionary ApplicationsMay 1, 2020Kevin M. Johnson, Morgan W. Kellydigested for all 80 samples in a 96‐well plate using the two frequent cutter enzymes AseI and NsiI (NEB‐R0127L and NEB‐R0526L; Van Gurp et al., 2016). Digested DNA was ligated to custom y‐yoked methylated sequencing

(6) Proteome Instability Is a Therapeutic Vulnerability in Mismatch Repair-Deficient CancerCancer cellMarch 16, 2020McGrail DJ, Garnett J, Yin J, Dai H, Shih DJH, Lam TNA, Li Y, Sun C, Li Y, Schmandt R, et al.Express,Invitrogen,Cat# 12605010 Bgll,New England Biolabs,Cat# R0144S Nsil,New England Biolabs,Cat# R0127S BseR1,New England Biolabs,Cat# R0581S Critical Commercial Assays,Critical Commercial Assays,Critical

(7) Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer CellsInternational Journal of Molecular SciencesFebruary 16, 2018Valentina Miano, Giulio Ferrero, Valentina Rosti, Eleonora Manitta, Jamal Elhasnaoui, Giulia Basile, Michele De Bortolidigestion will be done by adding a total of 450 U of restriction enzyme (BglII and NsiI, NEB R0144L and R0127L respectively) overnight at 37 ◦ C while shaking at 300 rpm. A 4.5 µL aliquot was taken by pipetting

(8) Fortilin binds IRE1α and prevents ER stress from signaling apoptotic cell deathNature communicationsMay 26, 2017Pinkaew D, Chattopadhyay A, King MD, Chunhacha P, Liu Z, Stevenson HL, Chen Y, Sinthujaroen P, McDougal OM, Fujise K.and Southern blotting (using radioactive DNA probes 1 and 2 after NdeI [R0111S, New England BioLabs] and NsiI [R0127S] digestion, respectively) for successful homologous recombination (Supplementary Fig. 4B). Two mutated

(9) Protein and Antibody Engineering by Phage DisplayMethods in EnzymologySeptember 3, 2016J.C. Frei, J.R. LaiRestriction enzymes: BssHII, BsiWI, NheI, NsiI, FseI (NEB, Ipswich, MA, catalog #s: R0199S, R0553S, R0131S, R0127S, R0558S, respectively) Alkaline Phosphatase, Calf Intestinal (NEB, Ipswich, MA, catalog #: M0290S)

(10) GC skew defines distinct RNA polymerase pause sites in CpG island promotersGenome ResearchNovember 1, 2015Wendy A. Kellner, Joshua S.K. Bell, Paula M. Vertinoenzymes used for the analysis of the MYC and SIAH2 loci were PstI (NEB no. R0140) and NsiI (NEB no. R0127); P2RY2 was XmaI (NEB no. R0180) and BsaWI (NEB no. R0567). The P2RY2 digest was modified slightly by

(11) CRISPR interference (CRISPRi) for sequence-specific control of gene expressionNature ProtocolsNovember 1, 2013Matthew H Larson, Luke A Gilbert, Xiaowo Wang, Wendell A Lim, Jonathan S Weissman, Lei S QiR3101S), BstXI (cat. no. R0113L), XhoI (cat. no. R0146S), DpnI (cat. no. R0176S) and NsiI (cat. no. R0127S) T4 DNA ligase (Roche, cat. no. 10481220001) Quick blunt-end DNA ligation kit (New England Biolabs,

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NsiI

NsiI from New England Biolabs

Sequence

New England Biolabs

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NsiI from New England Biolabs

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